(Click on each image for a larger picture)
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Slide 1. “Human Cheek Cell”.
An image of a human buccal epithelial cell obtained using Differential Interference Contrast (DIC) microscopy. The largest subcellular structure detected is the nucleus (large, rod shaped), which is surrounded by smaller organelles and vesicles. Bio 445: Cellular Imaging
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Slide 2: “Organization of F-actin in tissue culture cell”
The image on the left shows a tissue culture cell stained with phalloidin to reveal the distribution of F-actin. The image on the right shows the same cell with DIC microscopy. Bio 419: Advanced Cell Biology
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Slide 3: “Triple-labeled bovine epithelial cell”.
This cell was fixed and stained with phalloidin to reveal the F-actin distribution (GREEN), tubulin antibodies to reveal the microtubule distribution (RED), and Dapi to show the location of the nucleus (BLUE). Each image was acquired separately and then overlayed using Metamorph (Universal imaging). Bio 419: Advanced Cellular Biology
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Slide 4. “Microtubules in an epithelial cell”.
This cell was fixed and stained with tubulin antibodies (BLUE, PURPLE) to show the distribution of microtubules (MTs) and DAPI to show the location of the nucleus (GREEN). Note individual MTs extending to the cell periphery at the top of the cell. Bio 419: Advanced Cell Biology
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Slide 5. “Fluorescence/DIC overlay” .
This cell was stained with phalloidin to reveal the F-actin distribution (RED), DAPI to show the location of the nucleus (YELLOW). The fluorescent images were then overlayed on the DIC image using Metamorph (Universal Imaging). Bio 445: Cellular Imaging
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Slide 6. “Fluorescence/DIC overlay 2”.
This cell was stained with tubulin antibodies to show the MT distribution (RED), DAPI to show the location of the nucleus (BLUE), and DnaseI to show the distribution of G-actin (Green). Each fluorecent image was acquired separately and overlayed onto the DIC image. Bio 445: Cellular Imaging
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Slide 7. “Glial and Neuron Interactions”.
DIC image of a glial-like cell (Black arrow) and neuron (white arrow shows cell body of neuron). Filopodia and lamellipodia from the glial-like cell appear to contact the axon of the neuron. (See movie: “Glial cell contacts neuronal axon”). Bio 435: Developmental Neurobiology
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Slide 8. “Distribution of F-actin and g-actin in growth cones.”
Growth cones form at the end of axons during development and regeneration, guiding the axon to the appropriate target. The distribution of F-actin (RED) and g-actin (GREEN) was revealed by staining the neuron with phalloidin and DNAseI. Bio 435: Developmental Neurobiology
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Slide 9. “Effect of cadium on actin cytoskeleton in growth cones”
Neurons were treated with CdCl for 30 minutes prior to fixation and stained with phalloidin and DNAseI to reveal the F-actin (RED) and g-actin distribution. (GREEN) Bio 435: Developmental Neurobiology
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Slide 10: “Distribution of F-actin and g-actin in growth cones II”.
The distribution of F-actin (RED) and g-actin (BLUE) was revealed by staining the neuron with phalloidin and DNAseI. Bio 435: Developmental Neurobiology
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